Excerpt from The Journal of Experimental Zoology, Vol. 16
While studying the phenomena of regeneration and structural regulation in Bursaria, it became desirable to know something of the relations of this organism to its food and of the processes which the solid food material undergoes in its passage into and through the cell and in the elimination of residues. This paper aims to present the first part of these observations and to make a general survey of the relations of this organism to food, leading up to a more detailed study of these and certain other problems of the cell as found in this unicellular animal.
Bursaria was found to be much more favorable for the investigation of these phenomena than the smaller infusoria such as Paramecium. No study has heretofore been made of the relations of Bursaria to food, so that the facts herein presented are new. The present investigation attempts not only to determine qualitatively whether the relations to food are similar to those more or less known for Paramecium, Stentor, Vorticella and other infusoria, but more particularly to work out and express these relations in a more quantitative way than has been done heretofore. It was found that certain kinds of experimental tests such as those on the rate of digestion, could be made upon this form, which it would have been difficult or impossible to carry out on smaller unicellular organisms. Its very large size offers a singular opportunity for easy manipulation in many kinds of work. When in a clear medium it is readily visible to the naked eye at a distance of six or eight feet and individuals may be transferred singly with a pipette without the aid of any magnifying instruments.
Bursaria occurs not infrequently in cultures brought from ponds in the vicinity of Baltimore, though it is less common than many other Protozoa. It can readily be cultivated in large culture dishes in the laboratory. In this way I have had abundant material at my disposal for many months. The method of cultivation has been simply the inoculation of an infusion of timothy hay in tap water from the wild culture; by several inoculations at different times one usually succeeds in obtaining large numbers.
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